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KMID : 0357920090430030250
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Korean Journal of Pathology
2009 Volume.43 No. 3 p.250 ~ p.253
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Analysis of HPV-other Samples by Performing HPV DNA Sequencing
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Choi Yoo-Duk
Han Chang-Woo
Chung Woon-Jae
Jung Woon-Won
Lee Ji-Shin
Nam Jong-Hee
Lee Min-Cheol
Juhng Sang-Woo
Choi Ho-Sun
Park Chang-Soo
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Abstract
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Background: HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.
Methods: For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high- risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV- other samples of 209 patients who subsequently underwent cervical biopsy.
Results: For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.
Conclusions: HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.
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KEYWORD
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DNA chips, Papillomaviridae, Sequence analysis, Uterine cervix
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